解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.924-933.1974
更新日期:1974-10-01 00:00:00
abstract::Five urea-resistant and two heat-resistant mutants of poliovirus types 1 and 2 were isolated and their structural and nonstructural polypeptides compared to those of their wild-type, parental strains in an attempt to correlate mutant phenotypes with alterations in specific capsid polypeptides. Four of the seven mutant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.821-833.1974
更新日期:1974-10-01 00:00:00
abstract::Rickard's strain of feline leukemia virus (FeLV) contains two large glycoproteins and five smaller polypeptides of molecular weights 100,000 (gp >/= 100), 70,000 (gp70), 30,000 (p30), 21,000 (p21), 15,000 (p15), 11,200 (p11), and 10,000 (p10) when chromatographed on 6% agarose in the presence of 6 M guanidine hydrochl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.2.349-365.1974
更新日期:1974-08-01 00:00:00
abstract::Revertants of Kirsten sarcoma virus transformed nonproducer BALB/3T3 cells (KA31 cells) were isolated after exposing the transformed cells to 5-fluorodeoxyuridine at high cell density, or when suspended in methylcellulose. Revertants were also isolated by treating KA31 cells with the lectin, concanavalin A, which is m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.2.239-248.1974
更新日期:1974-08-01 00:00:00
abstract::Newcastle disease virus (NDV) California strain reportedly grows poorly in L cells but replicates very well in chicken embryo cells. NDV-infected L cell cultures show a characteristic virus growth curve with respect to uridine incorporation, but plaque assays of the virus produced 24 h postinfection (PI) show no infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.162-169.1974
更新日期:1974-07-01 00:00:00
abstract::A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
abstract::Goat and rabbit antisera prepared against a purified Rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in Rauscher and Friend virus preparations. Absorption studies revealed that most of the neutralizing activity of goat anti-Rauscher virus gp69/71 serum was directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.187-189.1974
更新日期:1974-07-01 00:00:00
abstract::When Epstein-Barr virus (EBV) negative cells (Raji) were treated with iododeoxyuridine, only the early antigen (EA) component was induced. There was no significant increase in EBV DNA and no virus particles were observed. Somatic-cell hybrids were prepared from the fusion of Raji and D98 cells (D98/Raji). When these c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.174-176.1974
更新日期:1974-07-01 00:00:00
abstract::The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.6.1254-1262.1974
更新日期:1974-06-01 00:00:00
abstract::Several independent cell lines transformed by simian virus 40 carry a species of viral RNA of 900,000 to 1,000,000 daltons. A viral RNA species of similar size is found early in the lytic cycle. Late in the viral lytic cycle, two prominent viral RNA species of about 600,000 and 900,000 daltons are seen. The larger lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.6.1263-1273.1974
更新日期:1974-06-01 00:00:00
abstract::Marek's disease virus DNA appears to be wound around a central structure connecting the two inner poles of the capsid. Based on electron micrographs of Marek's disease virions, a diagram of spatial configuration of virus DNA is presented. This diagram may explain the pleomorphic character of the herpesvirus core obser...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1148-1150.1974
更新日期:1974-05-01 00:00:00
abstract::The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1134-1142.1974
更新日期:1974-05-01 00:00:00
abstract::Utilizing the isolated left and right halves of both adenovirus type 2 and the nondefective adenovirus simian virus 40 hybrid (Ad2(+)ND(1)), studies were undertaken to find the site on the DNA molecules at which replication begins. The data are consistent with several models which include an initiation event at both e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1046-1054.1974
更新日期:1974-05-01 00:00:00
abstract::Early in infection by bacteriophage T4, before replication has commenced, one can detect the presence of newly synthesized DNA which cosediments with parental phage DNA on sucrose gradients. As shown earlier (R. E. Murray and C. K. Mathews, 1969), some of this represents covalent attachment of new material to parental...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.4.847-857.1974
更新日期:1974-04-01 00:00:00
abstract::A double-isotopic label difference analysis of polyacrylamide gels has been used to distinguish between cellular and viral protein accumulation in infected cells and to quantify the kinetics of accumulation of viral polypeptides. This technique, coupled with the determination of total radioactive amino acid incorporat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.4.788-800.1974
更新日期:1974-04-01 00:00:00
abstract::Antisera were prepared against the three structural polypeptides of HADEN virus dissociated with sodium dodecyl sulfate. These immunological reagents were used in immunofluorescence tests to study the kinetics and location of polypeptide antigen appearance in infected cells. These sera did not neutralize virus infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.608-613.1974
更新日期:1974-03-01 00:00:00
abstract::Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.706-711.1974
更新日期:1974-03-01 00:00:00
abstract::The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.677-689.1974
更新日期:1974-03-01 00:00:00
abstract::The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. Twelve pol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.411-418.1974
更新日期:1974-02-01 00:00:00
abstract::The folded genome of Escherichia coli is converted to a slower-sedimenting form within 5 min after infection with bacteriophage T4 or T4nd28(den A)-amN82(44). Chloramphenicol sensitivity and response to UV-irradiation of the phage suggest participation of viral-induced functions. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.548-550.1974
更新日期:1974-02-01 00:00:00
abstract::The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.400-410.1974
更新日期:1974-02-01 00:00:00
abstract::A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.340-346.1974
更新日期:1974-02-01 00:00:00
abstract::A temperature-sensitive mutant of adenovirus 31 was defective in formation of the DNA replication complex, suggesting the existence of a virus-coded protein necessary for the complex-formation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.538-540.1974
更新日期:1974-02-01 00:00:00
abstract::Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.42-45.1974
更新日期:1974-01-01 00:00:00
abstract::A lysate from hypotonically swollen polyoma-infected BALB/3T3 cells incorporated labeled deoxynucleotide triphosphates into both viral and cellular DNAs. The incorporation was stimulated by the presence of ATP, deoxynucleotide triphosphates, thiols, and magnesium ions. Strong inhibition of incorporation was observed w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.125-139.1974
更新日期:1974-01-01 00:00:00
abstract::Phage 2 adsorbed to Pseudomonas aeruginosa strain BI in 5 mM Tris buffer, providing that cations like Na(+), Mg(2+), or Ca(2+) were present. Adsorption was observed over a broad pH range, reaching a maximum level around pH 7.5, which coincided with the pH required for maximal activity of the phage 2-associated slime p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.22-27.1974
更新日期:1974-01-01 00:00:00
abstract::The synthesis of membrane protein after infection with bacteriophage T4 was examined. Protein constituents of both the cytoplasmic and outer membrane are made during the infective cycle. In addition, newly synthesized membrane protein is found in material which has a buoyant density greater than that of either of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.73-80.1974
更新日期:1974-01-01 00:00:00
abstract::A virus structurally similar to viruses associated with maedi, progressive pneumonia, and visna of sheep has been isolated from buffy coat cells of cattle with chronic lymphocytosis. Electron microscope studies revealed three variants of the virion: (i) an intracytoplasmic form 98 to 116 nm in diameter when occurring ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.197-204.1974
更新日期:1974-01-01 00:00:00
abstract::Antiserum against murine cytomegalovirus produced in the rabbit contained complement (C')-requiring neutralizing (CRN) antibody. The proportion of CRN was extremely high (up to 98%) during the early portion of an immunization procedure, whereas the antisera produced late had a much lower proportion that required C'. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1620-1621.1973
更新日期:1973-12-01 00:00:00
abstract::The endogenous transcriptase present in purified vesicular stomatitis (VS) virions was solubilized with a Triton X-100 high-salt solution. The polymerase activity was purified on glycerol gradients and by phosphocellulose column chromatography; the viral proteins present in the active enzyme fractions were identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1325-1335.1973
更新日期:1973-12-01 00:00:00
abstract::The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,00...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1503-1511.1973
更新日期:1973-12-01 00:00:00
abstract::It is shown that gene 5 protein of bacteriophage M13 is one of the major proteins synthesized in vitro under the direction of M13 replicative-form DNA. By means of DNA-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1466-1472.1973
更新日期:1973-12-01 00:00:00
abstract::Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and proceeds by a semiconservative mechanism. Both strands of the phage DNA are replicated into a high molecular weight proge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1195-1203.1973
更新日期:1973-12-01 00:00:00
abstract::Ultrastructure was correlated with growth kinetics of bdellophage VL-1 infecting host-dependent ("parasitic") Bdellovibrio bacteriovorus 109J in its Escherichia coli B host (the three-membered system), as well as in the host-independent ("saprophytic") derivative of the Bdellovibrio. Electron microscope observations s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1522-1533.1973
更新日期:1973-12-01 00:00:00
abstract::The DNA product of the endogenously instructed RNA-dependent DNA polymerase reaction of murine sarcoma virus continued to be synthesized for as long as 64 h in the presence of 0.008% Triton X-100. Higher detergent concentrations and actinomycin D inhibited DNA product synthesis. The DNA product from long-term polymera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.5.1049-1064.1973
更新日期:1973-11-01 00:00:00
abstract::DNA polymerase activity was detected in each of eight preparations of concentrated human hepatitis B antigen (HBAg) rich in Dane particles prepared by high-speed centrifugation of antigen-positive human plasma and in none of seven control preparations prepared in the same way from HBAg-negative plasma. The incorporati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.5.995-1005.1973
更新日期:1973-11-01 00:00:00
abstract::The covalent linkage of oncornavirus-specific DNA to chicken DNA was investigated in normal chicken embryo fibroblasts (CEF) and in virus-producing leukemic cells transformed by avian myeloblastosis virus (AMV). The virus-specific sequences present in cellular DNA fractionated by different methods were detected by DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.721-732.1973
更新日期:1973-10-01 00:00:00
abstract::DNA was extracted from [(3)H]thymidine-labeled Marek's disease virus (MDV) and purified by two cycles of CsCl gradient centrifugation in a fixed-angle rotor. The DNA was transcribed in vitro into (32)P-labeled complementary RNA (cRNA). MDV cRNA did not hybridize with DNA from chicken embryo fibroblast cultures or from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.841-846.1973
更新日期:1973-10-01 00:00:00
abstract::The cell culture lines HTG2 and HTG3 were established from a transplantable hamster tumor induced by a murine sarcoma virus (strain Gz-MSV) after 17 and 60 in vivo passages, respectively. The viruses released by these two cell lines markedly differ in morphology, antigenic composition, infectivity, transforming abilit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.931-936.1973
更新日期:1973-10-01 00:00:00
abstract::Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.625-631.1973
更新日期:1973-09-01 00:00:00